Membrane on a Chip: A Functional Tethered Lipid Bilayer Membrane on Silicon Oxide Surfaces

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Imaging Lipid Microdomains in a Microfluidic Bilayer Lipid Membrane Chip

A microfluidic bilayer lipid membrane (BLM) system supporting correlated opto-electrical membrane characterization has been developed and applied to the imaging of phase-separated lipid microdomains by confocal microscopy. The device consists of a microfluidic network supporting rapid perfusion fabricated in a thermoplastic substrate, bonded to a thin PVDC partition and supported by a thin cove...

متن کامل

Atomic force measurements on the specifically orientated membrane protein TGR5 reconstituted inside a tethered bilayer lipid membrane

Background The G-protein coupled bile acid receptor (GPBAR1), commonly known as TGR5 is a transmembrane protein associated with diabetes, metabolic syndrome, inflammation and cancer in various organs. Together with these diseases a TGR5 deficiency, overexpression or mutation can often be observed. Due to this association of TGR5 and its mutants with different disorders, TGR5 is seen as a potent...

متن کامل

An evidence for a potassium channel in endoplasmic reticulum based on single channel recording in bilayer lipid membrane

Introduction Numerous studies have demonstrated the presence of potassium selective channels in membranes internal organelles. These channels are essential to a large variety of cellular processes including intracellular 2+ a signaling, protein recycling, charge neutralization and cell protection. In contrast to the sarcoplasmic reticulum + here potassium channels have been clearly ...

متن کامل

A study of cytochrome bo3 in a tethered bilayer lipid membrane.

An assay has been developed in which the activity of an ubiquinol oxidase from Escherichia coli, cytochrome bo(3) (cbo(3)), is determined as a function of the hydrophobic substrate ubiquinol-10 (UQ-10) in tethered bilayer lipid membranes (tBLMs). UQ-10 was added in situ, while the enzyme activity and the UQ-10 concentration in the membrane have been determined by cyclic voltammetry. Cbo(3) is i...

متن کامل

The protein-tethered lipid bilayer: a novel mimic of the biological membrane.

A new concept of solid-supported tethered bilayer lipid membrane (tBLM) for the functional incorporation of membrane proteins is introduced. The incorporated protein itself acts as the tethering molecule resulting in a versatile system in which the protein determines the characteristics of the submembraneous space. This architecture is achieved through a metal chelating surface, to which histid...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2005

ISSN: 0006-3495

DOI: 10.1529/biophysj.105.061374